Maps sites for restriction enzymes, a.k.a. restriction endonucleases, in DNA sequences. Also does virtual digestion.
Conformation
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Maximum Cuts all 0 1 2 3 4 5 10 20 30 40 Minimum Site Length 4 5 6 7 8
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All Enzymes AarI AasI AatI AatII AccI AccII AccIII Acc16I Acc36I Acc65I Acc113I AccB1I AccB7I AccBSI AciI AclI AclWI AcsI AcuI AcvI AcyI AdeI AfaI AfeI AflII AflIII AgeI AhdI AhlI AleI AloI AluI AlwI Alw21I Alw26I Alw44I AlwNI Ama87I Aor51HI ApaI ApaLI ApoI AscI AseI AsiAI AsiSI AspI Asp700I Asp718I AspA2I AspEI AspHI AspLEI AspS9I AsuII AsuC2I AsuHPI AsuNHI AvaI AvaII AviII AvrII AxyI BaeI BalI BamHI BanI BanII BanIII BbeI BbrPI BbsI BbuI BbvI Bbv12I BbvCI BccI BceAI BcgI BciVI BclI BcnI BcuI BfaI BfiI BfmI BfrI BfrBI BfuI BfuAI BfuCI BglI BglII BlnI BlpI Bme18I Bme1390I Bme1580I BmgBI BmrI BmtI BmyI BoxI BpiI BplI BpmI Bpu10I Bpu14I Bpu1102I BpuAI BpuEI BsaI Bsa29I BsaAI BsaBI BsaHI BsaJI BsaMI BsaWI BsaXI Bsc4I Bse1I Bse8I Bse21I Bse118I BseAI BseBI BseCI BseDI Bse3DI BseGI BseJI BseLI BseMI BseMII BseNI BsePI BseRI BseSI BseXI BseX3I BseYI BsgI Bsh1236I Bsh1285I BshFI BshNI BshTI BsiEI BsiHKAI BsiHKCI BsiSI BsiWI BsiYI BslI BsmI BsmAI BsmBI BsmFI Bso31I BsoBI BsoMAI Bsp13I Bsp19I Bsp68I Bsp106I Bsp119I Bsp120I Bsp143I Bsp143II Bsp1286I Bsp1407I Bsp1720I BspCI BspCNI BspDI BspEI BspHI BspLI BspLU11I BspMI BspMAI BspPI BspTI BspT104I BspT107I BspTNI BspXI BsrI BsrBI BsrDI BsrFI BsrGI BsrSI BssAI BssECI BssHI BssHII BssKI BssNAI BssSI BssT1I Bst6I Bst98I Bst1107I BstACI BstAPI BstAUI BstBI Bst2BI BstBAI Bst4CI BstC8I BstDEI BstDSI BstEII BstENI BstENII BstF5I BstFNI BstH2I BstHHI BstHPI BstKTI BstMCI BstMWI BstNI BstNSI BstOI BstPI BstPAI BstSCI BstSFI BstSNI BstUI Bst2UI BstV1I BstV2I BstXI BstX2I BstYI BstZI BstZ17I Bsu15I Bsu36I BsuRI BsuTUI BtgI BtrI BtsI BveI Cac8I CaiI CciNI CelII CfoI CfrI Cfr9I Cfr10I Cfr13I Cfr42I ClaI CpoI CspI Csp6I Csp45I CspAI CviJI CviRI CviTI DdeI DpnI DpnII DraI DraII DraIII DrdI DseDI EaeI EagI Eam1104I Eam1105I EarI EciI Ecl136II EclHKI EclXI Eco24I Eco31I Eco32I Eco47I Eco47III Eco52I Eco57I Eco72I Eco81I Eco88I Eco91I Eco105I Eco130I Eco147I EcoICRI Eco57MI EcoNI EcoO65I EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI Esp3I FalI FatI FauI FauNDI FbaI FblI Fnu4HI FokI FriOI FseI FspI FspAI Fsp4HI FunI FunII GsuI HaeII HaeIII HapII HgaI HhaI Hin1I Hin4I Hin6I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI Hpy8I Hpy99I Hpy188I Hpy188III HpyCH4III HpyCH4IV HpyCH4V HpyF10VI Hsp92I Hsp92II HspAI ItaI KasI KpnI Kpn2I KspI Ksp22I Ksp632I KspAI Kzo9I LweI MabI MaeI MaeII MaeIII MamI MbiI MboI MboII MfeI MflI MhlI MlsI MluI MluNI MlyI Mly113I MmeI MnlI Mph1103I MroI MroNI MroXI MscI MseI MslI MspI Msp20I MspA1I MspCI MspR9I MssI MunI MvaI Mva1269I MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NmuCI NotI NruI NruGI NsbI NsiI NspI NspIII NspV OliI PacI PaeI PaeR7I PagI PalI PauI PceI PciI PctI PdiI PdmI Pfl23II PflBI PflFI PflMI PfoI PhoI PinAI PleI Ple19I PmaCI PmeI PmlI PpiI PpsI PpuMI PpuXI PshAI PshBI PsiI Psp5II Psp6I Psp1406I PspAI Psp124BI PspEI PspGI PspLI PspN4I PspOMI PspPI PspPPI PspXI PsrI PstI PsuI PsyI PvuI PvuII RcaI RsaI RsrII Rsr2I SacI SacII SalI SanDI SapI SatI Sau96I Sau3AI SbfI ScaI SchI ScrFI SdaI SduI SexAI SfaNI SfcI SfiI SfoI Sfr274I Sfr303I SfuI SgfI SgrAI SgrBI SinI SlaI SmaI SmiI SmiMI SmlI SmuI SnaBI SpaHI SpeI SphI SrfI Sse9I Sse8387I SseBI SspI SspBI SstI StuI StyI StyD4I SunI SwaI TaaI TaiI TaqI TaqII TasI TatI TauI TelI TfiI TliI Tru1I Tru9I TscI TseI Tsp45I Tsp509I TspDTI TspEI TspGWI TspRI Tth111I Van91I Vha464I VneI VpaK11BI VspI XagI XapI XbaI XceI XcmI XhoI XhoII XmaI XmaCI XmaJI XmiI XmnI XspI ZhoI ZraI Zsp2I All Commercial NEB only 5' overhang 3' overhang blunt Prototypes Only All Isoschizomers
Sequence Info
No non-base letters. Numbers and spaces OK. Paste Sequence Here Name your sequence
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